J.M. DE JESÚS-MIRANDA1
, L. A. MANDUJANO2
, F. MÉNDEZ3
, Y.J.
CASTILLO1
, J. MULIA1
, C. GARCÍA4
, Y.E. FELIPE4
AND D. OSORIOGONZÁLEZ1*
1 Laboratorio de Biofísica Molecular de la Facultad de Ciencias. Universidad Autónoma del Estado de México.
2Laboratorio de Diseño y Modelado Biofísico Molecular de la Universidad Mexiquense S. C.
3 Laboratorio de Ecofisiología Animal de la Facultad de Ciencias. Universidad Autónoma del Estado de México.
4 Laboratorio de Biología de la Reproducción de la Facultad de Medicina Veterinaria y Zootecnia. Universidad Autónoma del Estado de México
*Email: dog@uaemex.mx
Abstract:
Infertility is a world disease in which a couple is unable to achieve pregnancy. There are numerous parameters to determinate fertility; nevertheless, sperm motility is by consensus one of the most important attributes to evaluate male fertility. Contributions to a better understanding of this crucial parameter are imperative; hence, the aim of this investigation was to assess the effect of low-energy laser irradiation on sperm cell dynamics in thawed samples that were cryopreserved. We used a 405 nm blue laser beam to irradiate spermatic cells from rabbit inside a temperature-controlled dispersion chamber at 37 °C; then, we applied an image recognizing system to calculate individual sperm trajectories and velocities. We found that sperms raise its motility after irradiation suggesting that λ=405 nm is an optimal wavelength for spermatic photo-stimulation.
DOI: https://doi.org/10.15415/jnp.2017.51018
LINK: http://dspace.chitkara.edu.in/jspui/bitstream/1/878/1/51018_JNP_Osorio-Gonz%C3%A1lez.pdf
1 Laboratorio de Biofísica Molecular de la Facultad de Ciencias. Universidad Autónoma del Estado de México.
2Laboratorio de Diseño y Modelado Biofísico Molecular de la Universidad Mexiquense S. C.
3 Laboratorio de Ecofisiología Animal de la Facultad de Ciencias. Universidad Autónoma del Estado de México.
4 Laboratorio de Biología de la Reproducción de la Facultad de Medicina Veterinaria y Zootecnia. Universidad Autónoma del Estado de México
*Email: dog@uaemex.mx
Abstract:
Infertility is a world disease in which a couple is unable to achieve pregnancy. There are numerous parameters to determinate fertility; nevertheless, sperm motility is by consensus one of the most important attributes to evaluate male fertility. Contributions to a better understanding of this crucial parameter are imperative; hence, the aim of this investigation was to assess the effect of low-energy laser irradiation on sperm cell dynamics in thawed samples that were cryopreserved. We used a 405 nm blue laser beam to irradiate spermatic cells from rabbit inside a temperature-controlled dispersion chamber at 37 °C; then, we applied an image recognizing system to calculate individual sperm trajectories and velocities. We found that sperms raise its motility after irradiation suggesting that λ=405 nm is an optimal wavelength for spermatic photo-stimulation.
DOI: https://doi.org/10.15415/jnp.2017.51018
LINK: http://dspace.chitkara.edu.in/jspui/bitstream/1/878/1/51018_JNP_Osorio-Gonz%C3%A1lez.pdf
