Using Green Fluorescent Protein to Correlate Temperature and Fluorescence Intensity into Bacterial Systems

DOI

10.15415/jnp.2016.41005

AUTHORS

K. Beltrán, J. M. de Jesús-Miranda, J. A. Castro, L. A. Mandujano-Rosas, J. M. Paulin-Fuentes, D. Osorio-González

ABSTRACT

The unique and stunning spectroscopic properties of Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria, not to mention of its remarkable structural stability, have made it one of the most widely studied and used molecular tool in medicine, biochemistry, and cell biology. Its high fluorescent quantum yield is due to its chromophore, structure responsible of emitting green visible light when excited at 395 nm. Although it is noteworthy that there is enormous available information of the wonderful luminescent properties of GFP, the fact is that there are features and properties unexplored yet, particulary about its capabilities as molecular reporter in several biological processes. In this work, we used recombinant DNA technology to express the protein in bacteria; prepared the bacterial system both in liquid and solid media, and assembled an experimental set to expose those media to a laser beam; thereby we excited the protein chromophore and used emission spectroscopy in order to observe variations in fluorescence when the bacterial system is exposed to different temperatures.

KEYWORDS

green fluorescent protein; bacterial systems; bacterial temperature; spectroscopic properties

REFERENCES

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On the Equivalent Sources and Geometric Factor Calculation for a Circular Detection

DOI

10.15415/jnp.2016.41004

AUTHORS

Tony Viloria A., Luis Montiel, Laszlo Sajo-Bohus, Daniel Palacios

ABSTRACT

In all absolute measurements of the intensity of the radioactive materials and calibration of the detectors, it is essential the knowledge of the geometric efficiency. This work describes how to obtain the sources with different geometries and equal geometric efficiency (equivalent sources for geometric factor), corresponding to a linear, circumferential and circular homogeneous sources parallel to a circular detector. It is estimated the geometric factor of them by the Monte Carlo method. The results are compared with the published in the literature, thus confirming the validity of this method.

KEYWORDS

geometric factor, Monte Carlo method, equivalent source.

LINK:http://jnp.chitkara.edu.in/abstract.php?id=472#exactabstracts

REFERENCES

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X-Ray Fluorescence Analysis of Fine Atmospheric Aerosols from a Site in Mexico City

DOI

10.15415/jnp.2016.41003

AUTHORS

A. E. Hernández-López, J. Miranda, J. C. Pineda

ABSTRACT

A study was performed in the Winter of the year 2015 in a Southwestern site in the MAMC (Ciudad Universitaria), collecting PM2.5 samples with a MiniVol. As a part of wider study focused to fully characterize aerosols at this site, an X-ray Fluorescence (XRF) spectrometer (based on an Rh X-ray tube) built to analyze environmental samples, was used to characterize the sample set. A total of 16 elements (Al, Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, and Pb) were detected in most samples and mean concentrations were calculated. Cluster analysis was also applied to the elemental concentrations to find possible correlations among the elements.

KEYWORDS

XRF, atmospheric aerosols

REFERENCES

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Experimental Setups for Single Event Effect Studies

DOI

10.15415/jnp.2016.41002

AUTHORS

N. H. Medina, V. A. P. Aguiar, N. Added, F. Aguirre, E. L. A. Macchione, S. G. Alberton, M. A. G. Silve ira, J. Benfica, F. Vargas, B. Porcher

ABSTRACT

Experimental setups are being prepared to test and to qualify electronic devices regarding their tolerance to Single Event Effect (SEE). A multiple test setup and a new beam line developed especially for SEE studies at the São Paulo 8 UD Pelletron accelerator were prepared. This accelerator produces proton beams and heavy ion beams up to 107Ag. A Super conducting Linear accelerator, which is under construction, may fulfill all of the European Space Agency requirements to qualify electronic components for SEE.

KEYWORDS

Radiation effects, electronic devices, single event effects.

LINK: http://jnp.chitkara.edu.in/abstract.php?id=470#exactabstracts

REFERENCES

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Revisiting Natural Radiation in Itacaré and Guarapari Beaches

AUTHORS

M. A. G. Silveira, J. M. Oliveira, V A. P. Aguiar, N. H. Medina

ABSTRACT

Human beings are constantly exposed to several types of natural radiation. This paper aims to study the total external dose from northwestern Brazilian beach sands. The samples were collected at Prainha in Itacaré, Bahia, and Praia de AreiaPreta in Guarapari, Espírito Santo. Gamma spectrometry is a very useful technique to estimate the effective dose due to naturally occurring radionuclides, such as 40K and daughters of 238U and 232Th. In order to confirm the high activity present in these two regions, the effective dose due to each natural radionuclide was determined. Moreover, the Energy-Dispersive X-Ray Spectroscopy (EDS) microanalysis was used to characterize the soil composition and the minerals responsible for the high activity. In addition, the sand samples were separated in to magnetic and non-magnetic fractions in order to identify the contribution from each portion of the activity. Finally, the radionuclides and their dispersion in those places are consistent with previous studies, indicating effective doses above the world average that is between 0.3 mSv/year and 1.0 mSv/year.

KEYWORDS

Natural radionuclides, beach sands, gamma-ray spectrometry.

LINK: http://jnp.chitkara.edu.in/abstract.php?id=469#exactabstracts

REFERENCES

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